Structure Of Dna And Rna

The relationship between the melting temperature ( ) of a long double-stranded molecule and its content is given by the empirical formula (often assumed at a standard ionic strength):

$ \text{Tm} ^\circ \text{C} \% \text{GC} \text{Tm} = 85^\circ \text{C} 5000 \text{ bp} \text{1. Calculate \%GC} \text{Tm} \text{2. Calculate \%AT} \text{A-T} \text{G-C} \text{3. Rounding} $