Biochemical Separation Techniques

Step 1: Understanding the techniques and their applications.
- Nuclear magnetic resonance spectroscopy (NMR) is used to study the structure of proteins (P-4).

- Fluorescence resonance energy transfer (FRET) measures proximity between specific sites on the protein (Q-1).

- Ultraviolet absorption spectroscopy helps determine the concentration of the protein by measuring absorption at specific wavelengths (R-2).

- Dynamic light scattering (DLS) is used to measure the size of proteins and particles (S-3).

Step 2: Analyzing the options.
(A) P-3, Q-1, R-2, S-4: Incorrect — This does not match the correct pairings.
(B) P-4, Q-1, R-2, S-3: Correct — This correctly matches the techniques with their applications.
(C) P-4, Q-3, R-1, S-2: Incorrect — The pairings do not match.
(D) P-3, Q-4, R-1, S-2: Incorrect — The pairings are incorrect.

Step 3: Conclusion.
The correct answer is (B) P-4, Q-1, R-2, S-3.