Structure Of Dna And Rna

Given below are two statements, one is labelled as Assertion (A) and the other is labelled as Reason (R):
Assertion (A): Only a few specific strains of E.coli are routinely used in recombinant DNA technology.
Reason (R): E.coli divides rapidly under laboratory conditions than their native counterparts.
In the light of the above statements, choose the most appropriate answer from the options given below

Which one of the following methods can be used for detecting DNA-protein interaction?

In a PCR, the reaction tubes are incubated first at 94 °C for 3 minutes, at 58 °C for 1 minute and at 72 °C for 2 minutes. What is the sequence of processes happening inside the tubes containing DNA template, dNTPs and Taq DNA polymerase?

Given below are two statements, one is labelled as Assertion (A) and the other is labelled as Reason (R):
Assertion (A): DNA fragments from any source can be cloned into a plasmid.
Reason (R) : A plasmid can be transferred to other cells by gene transfer mechanism such as transformation and transduction.
In the light of the above statements, choose the most appropriate answer from the options given below

Given below are two statements, one is labelled as Assertion (A) and the other is labelled as Reason (R):
Assertion (A) : DNA is a stable genetic material.
Reason (R) : DNA is double-stranded only.
In the light of the above statements, choose the most appropriate answer from the options given below

Based on the context given below, answer the following questions:
DNA in eukaryotes is a double-helical structure with two anti-parallel strands intertwined equidistantly. The double-helical structure was deciphered by James Watson and Francis Crick on the basis of X-ray diffraction studies. The hydrophilic backbones of alternating sugar and phosphate groups are on the outside of the helix held together by phosphodiester bonds. The purine and pyrimidine bases of both the strands are stacked inside the double helix. Adenine binds with thymine with hydrogen bond and cytosine binds with guanine with hydrogen bond. The DNA is transcribed into messenger RNA, ribosomal RNA and transfer RNA. Messenger RNA is translated into protein in the cytoplasm.

In eukaryotes, 5S rRNA is synthesized by

The method used to analyse larger DNA fragments (>1 Mbp) is

Cosmid vector is made up of :

Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R.
Assertion A: During gel electrophoresis, DNA moves from anode to cathode.
Reason R: DNA is negatively charged.
In the light of the above statements, choose the most appropriate answer from the options given below :

Arrange the steps involved in cloning of gene into plasmid:
A. Selection of confirmation of recombinant clones
B. Ligation of the gene of interest and plasmid
C. Amplification of the gene of interest using template DNA
D. Transformation of bacterium using the recombinant plasmid
E. Restriction endonuclease treatment of the suitable plasmid and gene of interest.
Choose the correct answer from the options given below :

Choose the correct sequence involved in transcription and translation process in eukaryotes:
A. mRNA leaves the nucleus and attaches to a ribosome in cytosol
B. RNA processing
C. Transcription
D. Peptic bond formation between amino acids
E. Ribosome assembly
Choose the correct answer from the options given below :

Match List I with List II

List I Enzymes		List II Functions
A.	DNA polymerase α (alpha)	I.	Base-excision DNA repair
B.	DNA polymerase ẞ (beta)	II.	RNA/DNA primary initiation of DNA synthesis
C.	polymerase γ (gamma)	III.	Mitochondrial DNA replication and repair
D.	DNA polymerase R (Kappa)	IV.	Translation DNA synthesis

Choose the correct answer from the options given below :

Given below are two statements:
Statement I: Messenger RNA capping, polyadenylation and splicing are coupled to transcription in eukaryotes.
Statement II: Transcription and translation are compartmentalized in prokaryotes.
In the light of the above statements, choose the correct answer from the options given below :

The anti-parallel nature of double standard DNA is due to :