Recombinant Dna Technology And Applications

The steps in recombinant DNA technology occur in the following order:
1. Isolation of DNA (D): The DNA is isolated from the source organism.
2. Digestion by restriction enzyme (A): Specific restriction enzymes are used to cut the DNA at desired sites.
3. Ligation (C): The DNA fragments are joined together using DNA ligase.
4. Transformation (B): The recombinant DNA is introduced into a host organism for replication and expression.
This sequence ensures that the recombinant DNA is properly prepared and successfully introduced into the host.