Dna Fingerprinting

Select the correct sequence of steps in DNA fingerprinting involving southern blot hybridization using radiolabelled VNTR as a probe. I. Hybridization using labelled VNTR probe. II. Isolation of DNA. III. Transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon. IV. Detection of hybridized DNA fragments by autoradiography. V. Separation of DNA fragments by electrophoresis. VI. Digestion of DNA by restriction endonucleases.

Microsatellites are