Genetic Disorders

A - Trypsin: In the first step, the proteins are treated with trypsin. Trypsin is a proteolytic enzyme that is used to cut proteins at specific amino acid sequences.

B - Paper Electrophoresis: The next step is to use paper electrophoresis to separate the resulting fragments based on their charge.

C - Sequencing: Finally, sequencing is done on the non-overlapping and eluted peptides from the chromatography, and this allows us to find the amino acid sequence of each peptide.