Biotechnology
17 previous year questions.
High-Yield Trend
Chapter Questions 17 MCQs
The cutting of DNA by restriction endonucleases results in the fragments of DNA. These fragments can be separated by a technique known as gel electrophoresis. Since DNA fragments are negatively charged molecules they can be separated by forcing them to move towards the anode under an electric field through a medium/ matrix. The commonly used matrix is agarose which is a natural polymer extracted from sea weeds. The DNA fragments separate according to their size through sieving effect provided by the agarose gel. Hence, the smaller fragments move farther in the agarose gel. The separated DNA fragments can be visualised only after staining the DNA with ethidium bromide followed by exposure to UV radiation. Bright orange coloured bands of DNA can be observed. The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution.
The DNA fragments purified in this way are used in constructing recombinant DNA by joining them with cloning vectors. Agarose is extracted from __ .
Bacteria
Marine animals
Sea weeds
Fungi
The cutting of DNA by restriction endonucleases results in the fragments of DNA. These fragments can be separated by a technique known as gel electrophoresis. Since DNA fragments are negatively charged molecules, they can be separated by forcing them to move towards the anode under an electric field through a medium/ matrix. The commonly used matrix is agarose which is a natural polymer extracted from sea weeds. The DNA fragments separate according to their size through sieving effect provided by the agarose gel. Hence, the smaller fragments move farther in the agarose gel. The separated DNA fragments can be visualised only after staining the DNA with ethidium bromide followed by exposure to UV radiation. Bright orange coloured bands of DNA can be observed. The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution. The DNA fragments purified in this way are used in constructing recombinant DNA by joining them with cloning vectors.
The DNA fragments formed by treatment with endonuclease are separated by.
PCR
Gel Electrophoresis
Cloning
Restriction digestion
The cutting of DNA by restriction endonucleases results in the fragments of DNA. These fragments can be separated by a technique known as gel electrophoresis. Since DNA fragments are negatively charged molecules, they can be separated by forcing them to move towards the anode under an electric field through a medium/ matrix. The commonly used matrix is agarose which is a natural polymer extracted from sea weeds. The DNA fragments separate according to their size through sieving effect provided by the agarose gel. Hence, the smaller fragments move farther in the agarose gel. The separated DNA fragments can be visualized only after staining the DNA with ethidium bromide followed by exposure to UV radiation. Bright orange coloured bands of DNA can be observed. The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution. The DNA fragments purified in this way are used in constructing recombinant DNA by joining them with cloning vectors. The separated DNA fragments can be visualized after staining with __ , followed by exposure to __.
B-galactosidase, UV radiation
B-galactosidase, Gamma radiation
Ethidium bromide, UV radiation
Ethidium bromide, Gamma radiation
The cutting of DNA by restriction endonucleases results in the fragments of DNA. These fragments can be separated by a technique known as gel electrophoresis. Since DNA fragments are negatively charged molecules, they can be separated by forcing them to move towards the anode under an electric field through a medium/ matrix. The commonly used matrix is agarose which is a natural polymer extracted from sea weeds. The DNA fragments separate according to their size through sieving effect provided by the agarose gel. Hence, the smaller fragments move farther in the agarose gel.
The separated DNA fragments can be visualised only after staining the DNA with ethidium bromide followed by exposure to UV radiation. Bright orange coloured bands of DNA can be observed. The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution. The DNA fragments purified in this way are used in constructing recombinant DNA by joining them with cloning vectors.
The fragments of DNA separate on agarose gel, based on the.
Size of fragments
Charge of each fragment
Colour of fragment
Type of DNA
List - I | List - II | ||
| A | Streptokinase | I | Blood-Cholestrol lowering agents |
| B | Cyclosporin | II | Clot Buster |
| C | Statins | III | Propionibacterium sharmanii |
| D | Swiss Cheese | IV | Immuno suppressive agent |
(A) - (II), (B) - (IV), (C) - (III), (D) - (I)
(A) - (II) (B) - (IV), (C) - (I), (D) - (III)
(A) -(IV), (B)- (I), (C)- (II) (D) - (III)
(A) -(IV), (B) - (I), (C) - (III), (D) - (I)
Which enzymes are used for clarification of bottled fruit juices?
(A) only
(A) and (B) only
(B) and (C) only
(C) and (D) only
The term “Clone” is used to describe the offspring that are :
Morphologically identical only
Morphologically and genetically identical
Morphologically identical but genetically different
Genetically identical only
‘Golden rice” variety of rice shows :
enhanced nutritional value of food
less post harvest loss
tolerence to abiotic stress
pest resistance
Which of the following is NOT an application of PCR ?
Paternity testing
Detection of mutations of genes in suspected cancer patient
Powerful technique to identify genetic disorder
To cure ADA (adenosine diaminase) deficiency
A. They should have ability to replicate within the host
B. They should have ‘ori’ sites that start replication
C. Its origin should not support high copy number
D. They should have more than one recognition sites for restriction enzymes
Choose the correct answer from the options given below :
(A) Temperature induced denaturation of double stranded DNA.
(B) Isolation of gene of interest
(C) Amplification of gene by using Taq polymerase
(D) Primer annealing on the separated templates
Choose the correct answer from the options given below:
LIST I | LIST II | ||
| A | First Restriction Endonuclease | I | GAATTC |
| B | Disarmed Plasmid | II | Selectable markers |
| C | Antibiotic resistant genes | III | Hind II |
| D | EcoRI | IV | Vector |
| List-I(Microbes) | List-II (Acid Produced) |
| (A) Aspergillus | (I) Lactic acid |
| (B) Acetobacter | (II) Butyric acid |
| (C) Clostridium | (III) Acetic acid |
| (D) Lactobacillus | (IV) Citric acid |