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AP-EAPCET SERIES
Botany

Applications Of Biotechnology

15 previous year questions.

Volume: 15 Ques
Yield: Medium

High-Yield Trend

2
2025
13
2023

Chapter Questions
15 MCQs

01
PYQ 2023
medium
botany ID: ap-eapce
Assertion (A): Bioterrorism involves the actual use or threat to use biological agents to spread fear or inflict death and death of large populations.
Reason (R): It paves way for the development of new vaccines and therapies.
1

Both A and R are correct and R is the correct explanation of A.

2
Both A and R are correct but R is not the correct explanation of A.
3
A is correct but R is incorrect.
4
A is incorrect but R is correct.
02
PYQ 2023
medium
botany ID: ap-eapce
This committee of Indian Government takes decisions while introducing genetically modified organisms for public services.
1
Genome Engineering Action Committee
2
Ground Environment Action Committee
3
Genetic Engineering Approval Committee
4
Genetic and Environment Approval Committee
03
PYQ 2023
medium
botany ID: ap-eapce

Study the below table and answer A, B, C

1
A: 20216, B: 29, C: 1986
2
A: 200000, B: 27, C: 1997
3
A: 10000, B: 34, C: 1958
4
A: 15996, B: 25, C: 1998
04
PYQ 2023
medium
botany ID: ap-eapce
Choose the correct pair for artificially restructured E. coli cloning vector
I. pBR322 - Boliver and Rodrigues - Natural Vector
II. Selectable marker - Antibiotics - amp", terR
III. Restriction sites - Hind III, Pst I - Link alien DNA
IV. roP - Proteins - supports high copy number
1
I & IV
2
II & III
3
III & IV
4
II & IV
05
PYQ 2023
medium
botany ID: ap-eapce
Parbhani Kranti is a variety of this plant and resistant to this
1
Sugar cane, red rot
2
Abelmoschus esculentus, bacterial blight
3
Cow pea, bacterial blight
4
Abelmoschus esculentus, Yellow mosaic virus
06
PYQ 2023
medium
botany ID: ap-eapce
These are having the ability to replicate within the bacterial cell, independent of control of chromosomal DNA.
1
Plasmids and virus
2
Plasmids and bacteria
3
Plasmids and bacteriophage
4
Nucleus and plasmids
07
PYQ 2023
easy
botany ID: ap-eapce
Choose the wrong pair corresponding to selection of transformed cells
08
PYQ 2023
medium
botany ID: ap-eapce
Assertion (A): Bio piracy is use of bio resources by multinational companies with proper authorization from the other countries and people.
Reason (R): Industrialized nations are poor at biodiversity but rich financially.
1
Both A and R are correct and R is the correct explanation of A.
2
Both A and R are correct but R is not the correct explanation of A.
3
A is correct but R is incorrect.
4
A is incorrect but R is correct.
09
PYQ 2023
medium
botany ID: ap-eapce
Among the following, the wrong statement related to Bt cotton
1
Bt toxin protein exists as inactive protoxins
2
The activated toxin binds to the surface of mid gut epithelial cells of insects
3
Protoxins are converted to form active toxin due to acidic pH
4
Bt toxins are insect group specific
10
PYQ 2023
medium
botany ID: ap-eapce
The transgenic Flavr Savr tomato carries cloned genes for
A. delay in ripening process
B. longer shelf life
C. added flavors
D. vitamin A
1
A & D
2
A & B
3
A & C & D
4
A & B & C
11
PYQ 2023
medium
botany ID: ap-eapce
Identify the correct combination.
A. Agrobacterium tumefaciens – vector for transgenic plant.
B. Escherichia coli – selectable marker.
C.Thermus aquaticus – Taq polymerase.
D.Bacillus thuringiensis – BT. Toxin.
1
A B D
2
A B C
3
A B C D
4
B C D
12
PYQ 2023
medium
botany ID: ap-eapce
The introduction of nematode-specific genes into host using Agrobacterium vectors causes the following action:
I. Formation of dsRNA.
II. Production of sense and antisense RNAs in the host cells.
III. RNA intermediate not formed.
IV. Silencing of specific mRNA of the nematode.
1
II, III, IV
2
I, II, III
3
I, III, IV
4
I, II, IV
13
PYQ 2023
medium
botany ID: ap-eapce

Mention the Nitrogen base sequence name, restriction cut sites and type of cut ends in the above DNA fragment.
5' - GAATTC - 3'
3' - CTTAAG - 5'

1
Recognition sequence, A & A, cohesive ends
2
Palindrome sequence, G & A, sticky ends
3
Endonuclease, T & T, staggered cut
4
Ligase sites, C & T, sticky ends
14
PYQ 2025
medium
botany ID: ap-eapce
Which of the following is not a biosafety concern for GM crops?
1
Transferring allergens to humans
2
Harmful effects on biodiversity
3
Change of fundamental nature of vegetable
4
Unauthorised use of bioresources by multinational companies
15
PYQ 2025
medium
botany ID: ap-eapce
Choose the incorrect statements:

I. Human insulin is made in fungus
II. Insulin DNA sequences are introduced into the genophore of E. coli
III. Recombinant E. coli produces insulin chains
1
II, III
2
I only
3
III only
4
I, II

About Applications Of Biotechnology - AP-EAPCET

Applications Of Biotechnology is a vital chapter for AP-EAPCET aspirants. Mastering the concepts covered in this chapter is essential for securing a top rank.

By rigorously practicing the previous year questions associated with this chapter, you can identify high-yield topics, understand the examiner's perspective, and boost your confidence during the actual exam.

Frequently Asked Questions

Why focus on Applications Of Biotechnology PYQs?

Analyzing PYQs for this specific chapter reveals the most frequently tested concepts and the typical complexity of questions, allowing you to tailor your study plan efficiently.

How to best use this analysis?

Review the topic breakdown to see which sub-topics within Applications Of Biotechnology carry the most weight. Then, tackle the questions iteratively to solidify your understanding.