Med Applications Humulin

With regard to insulin, choose the correct options:

(a)	C-peptide is not present in mature insulin.
(b)	The insulin produced by rDNA technology has C-peptide.
(c)	The pro-insulin has C-peptide.
(d)	A-peptide and B-peptide of insulin are interconnected by disulphide bridges.

Choose the correct answer from the options given below:

1.	(a), (c) and (d) only	2.	(a) and (d) Only
3.	(b) and (d) only	4.	(b) and (c) only

For effective treatment of the disease, early diagnosis and understanding of its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection?

1.	ELISA Technique	2.	Hybridization Technique
3.	Western Blotting Technique	4.	Southern Blotting Technique

Statements related to human Insulin are given below.
Which statement(s) is/are correct about genetically engineered Insulin?

(a)	Pro-hormone insulin contain extra stretch of C-peptide
(b)	A-peptide and B-peptide chains of insulin were produced separately in E.coli, extracted and combined by creating disulphide bond between them.
(c)	Insulin used for treating Diabetes was extracted from Cattles and Pigs.
(d)	Pro-hormone Insulin needs to be processed for converting into a mature and functional hormone.
(e)	Some patients develop allergic reactions to the foreign insulin.

Choose the most appropriate answer from the options given below:

1.	(c), (d) and (e) only	2.	(a), (b) and (d) only
3.	(b) only	4.	(c) and (d) only

Which of the following genetically engineered organisms was used by Eli Lilly to prepare human insulin?

1.	Virus	2.	Phage
3.	Bacterium	4.	Yeast