Restriction Enzymes Main Enzymes

Restriction endonuclease:

1. cuts the DNA molecule randomly

2. cuts the DNA molecule at specific sites

3. restricts the synthesis of DNA inside the nucleus

4. synthesis DNA

Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?

1.	5'-CGTTCG-3'	2.	5'-GATATG-3'
	3'-ATGGTA-5'		3'-CTACTA-5'
3.	5'-GAATTC-3'	4.	5'-CACGTA-3'
	3'-CTTAAG-5'		3'-CTCAGT-5'

Restriction endonucleases are enzymes which:

1.	make cuts at specific positions within the DNA molecule
2.	recognize a specific nucleotide sequence for binding of DNA ligase
3.	restrict the action of the enzyme DNA polymerase
4.	remove nucleotides from the ends of the DNA molecule

Which of the following restriction enzymes produces blunt ends?

1. Sal l

2. Eco RV

3. Xho

4. Hind lll

Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement:

1.	The enzyme recognizes a specific palindromic nucleotide sequence in the DNA
2.	The enzyme cuts DNA molecules at an identified position within the DNA
3.	The enzyme binds DNA at specific sites and cuts only one of the two strands
4.	The enzyme cuts the sugar-phosphate backbone at specific sites on each strand

A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:

1.	Palindromic Nucleotide sequences	2.	Poly(A) tail sequences
3.	Degenerate primer sequence	4.	Okazaki sequences

In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme?
1. 5'GTATTC3' ; 3'CATAAG5'
2. 5'GATACT3' ; 3'CTATGA5'
3. 5'GAATTC3' ; 3'CTTAAG5'
4. 5'CTCAGT3' ; 3'GAGTCA5'

Give below are two statements:

Statement I:	Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence.
Statement II:	Restriction endonucleases cut the DNA strand a little away from the centre of the Palindromic site.

In the light of the above statements, choose the most appropriate answer from the options given below:
1. Statement I is incorrect but Statement II is correct.
2. Both Statement I and Statement II are correct.
3. Both Statement I and Statement II are incorrect.
4. Statement I is correct but Statement II is incorrect.

Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:

1.	6 bp	2.	4 bp
3.	10 bp	4.	8 bp

Given below are two statements :

Statement I :	The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.
Statement II :	Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.

In the light of the above statements, choose the most appropriate answer from the options given below :

1.	Statement I is correct but statement II is incorrect
2.	Statement I is incorrect but statement II is correct
3.	Both statement I and statement II are correct
4.	Both statement I and statement II are incorrect