Separation And Isolation Of Dna Fragments
High-Yield Trend
Questions 9 MCQs
1. tissue culture
2. PCR
3. gel electrophoresis
4. spectrophotometry
DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by:
1. Polymerase chain reaction
2. Electrophoresis
3. Restriction mapping
4. Centrifugation
The DNA fragments separated on an agarose gel can be visualized after staining with:
| 1. | Acetocarmine | 2. | Aniline blue |
| 3. | Ethidium bromide | 4. | Bromophenol blue |
DNA fragments are:
1. Negatively charged
2. Neutral
3. Either positively charged depending on their size
4. Positively charged
What is the criterion for the movement of DNA fragments on agarose gel during gel electrophoresis?
| 1. | The smaller the fragment size, the farther it moves |
| 2. | Positively charged fragments move to farther end |
| 3. | Negatively charged fragments do not move |
| 4. | The larger the fragment size, the farther it moves |
In a mixture, DNA fragments are separated by-
1. Bioprocess engineering
2. Restriction digestion
3. Electrophoresis
4. Polymerase chain reaction
DNA strands on a gel, stained with ethidium bromide, when viewed under UV radiation, appear as:
| 1. | Dark red bands | 2. | Bright blue bands |
| 3. | Yellow bands | 4. | Bright orange bands |
| 1. | Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light. |
| 2. | The process of extraction of separated DNA strands from gel is called elution. |
| 3. | The separated DNA fragments are stained by using ethidium bromide. |
| 4. | The presence of chromogenic substrate gives blue coloured DNA bands on the gel. |
| 1. | Bright orange colour | 2. | Bright red colour |
| 3. | Bright Blue colour | 4. | Bright yellow colour |
Preparing Separation And Isolation Of Dna Fragments for NEET
Separation And Isolation Of Dna Fragments is a specific sub-topic that frequently appears in the NEET examination. Understanding the underlying principles and practicing targeted questions is key to mastering this concept.
The questions compiled above are previous year questions (PYQs) directly related to Separation And Isolation Of Dna Fragments. Practicing these specific questions helps you understand the difficulty level and the examiner's approach to this topic.
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