NEET Zoology
Separation And Isolation Of Dna Fragments
Low Priority
Consistency: 35%
Weightage: 7 / 20 Yrs
High-Yield Trend
1
2023 1
2022 1
2021 1
2020 3
2017 1
2013 1
2011 Questions 9 MCQs
01
PYQ 2011
easy
zoology ID: neet-201
Agarose extracted from seaweeds finds use in:
1. tissue culture
2. PCR
3. gel electrophoresis
4. spectrophotometry
1. tissue culture
2. PCR
3. gel electrophoresis
4. spectrophotometry
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02
PYQ 2013
easy
zoology ID: neet-201
DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by:
1. Polymerase chain reaction
2. Electrophoresis
3. Restriction mapping
4. Centrifugation
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03
PYQ 2017
easy
zoology ID: neet-201
The DNA fragments separated on an agarose gel can be visualized after staining with:
| 1. | Acetocarmine | 2. | Aniline blue |
| 3. | Ethidium bromide | 4. | Bromophenol blue |
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04
PYQ 2017
easy
zoology ID: neet-201
DNA fragments are:
1. Negatively charged
2. Neutral
3. Either positively charged depending on their size
4. Positively charged
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05
PYQ 2017
easy
zoology ID: neet-201
What is the criterion for the movement of DNA fragments on agarose gel during gel electrophoresis?
| 1. | The smaller the fragment size, the farther it moves |
| 2. | Positively charged fragments move to farther end |
| 3. | Negatively charged fragments do not move |
| 4. | The larger the fragment size, the farther it moves |
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06
PYQ 2020
easy
zoology ID: neet-202
In a mixture, DNA fragments are separated by-
1. Bioprocess engineering
2. Restriction digestion
3. Electrophoresis
4. Polymerase chain reaction
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07
PYQ 2021
easy
zoology ID: neet-202
DNA strands on a gel, stained with ethidium bromide, when viewed under UV radiation, appear as:
| 1. | Dark red bands | 2. | Bright blue bands |
| 3. | Yellow bands | 4. | Bright orange bands |
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08
PYQ 2022
easy
zoology ID: neet-202
Which one of the following statement is not true regarding gel electrophoresis technique?
| 1. | Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light. |
| 2. | The process of extraction of separated DNA strands from gel is called elution. |
| 3. | The separated DNA fragments are stained by using ethidium bromide. |
| 4. | The presence of chromogenic substrate gives blue coloured DNA bands on the gel. |
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09
PYQ 2023
easy
zoology ID: neet-202
Upon exposure to UV radiation, DNA stained with ethidium bromide will show:
| 1. | Bright orange colour | 2. | Bright red colour |
| 3. | Bright Blue colour | 4. | Bright yellow colour |
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