Process Of Biotech

Spooling is:

1.	Amplification of DNA
2.	Cutting of separated DNA bands from the agarose gel
3.	Transfer of separated DNA fragments to synthetic membranes
4.	Collection of isolated DNA

Select the correct statement from the following:

1.	Gel electrophoresis is used for the amplification of a DNA segment.
2.	The polymerase enzyme joins the gene of interest and the vector DNA.
3.	Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions.
4.	PCR is used for the isolation and separation of genes of interest.

Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.

A.	Insertion of recombinant DNA into the host cell.
B.	Cutting of DNA at specific location by restriction enzyme.
C.	Isolation of desired DNA fragment.
D.	Amplification of gene of interest using PCR.

Choose the correct answer from the options given below:

1.	B, D, A, C	2.	B, C, D, A
3.	C, A, B, D	4.	C, B, D, A