Tools Vectors Ii

The figure below is the diagrammatic representation of the E.coli vector pBR 322. Which one of the given options correctly identifies its certain components (s)?

1. Ori-original restriction enzyme
2. Rop-reduced osmotic pressure
3. Hind III, Eco RI-selectable markers
4. Amp^R, teti^R-antibiotic resistance genes

The introduction of t-DNA into plants involves?

1. Infection of the plant by Agrobacterium tumefaciens

2. Altering the pH of the soil, heat-shocking the plants

3. Exposing the plants to Cold for a brief period

4. Allowing the plant roots to stand in water