Polymerase Chain Reaction Pcr

Which one is a true statement regarding DNA polymerase used in PCR?

1.	It is used to ligate introduces DNA in recipient cells
2.	It serves as a selectable marker
3.	It is isolated from a virus
4.	It remains active at high temperatures

Match the following techniques or instruments with their usage:

(a)	Bioreactor	(i)	Separation of DNA fragments
(b)	Electrophoresis	(ii)	Production of large quantities of products
(c)	PCR	(iii)	Detection of pathogen, based on antigen-antibody reaction
(d)	ELISA	(iv)	Amplification of nucleic acids

Select the correct option from the following:

1.	(a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)
2.	(a)-(ii), (b)-(i), (c)-(iv), (d)-(iii)
3.	(a)-(iv), (b)-(iii), (c)-(ii), (d)-(i)
4.	(a)-(ii), (b)-(i), (c)-(iii), (d)-(iv)

Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?

1. Extension, Denaturation, Annealing

2. Annealing, Denaturation, Extension

3. Denaturation, Annealing, Extension

4. Denaturation, Extension, Annealing

During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following PCR will be affected first?

1.	Denaturation	2.	Ligation
3.	Annealing	4.	Extension

Polymerase chain reaction (PCR) amplifies DNA following the equation.

1.		2.
3.		4.