Cloning Vector
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Questions 4 MCQs
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for -galactoside production and the recombinant plasmid is inserted in an E.coli strain,
| 1. | it will lead to the lysis of host cells. |
| 2. | it will be able to produce a novel protein with dual abilities. |
| 3. | it will not be able to confer ampicillin resistance to the host cell. |
| 4. | the transformed cells will have the ability to resist ampicillin as well as produce \beta -galactoside. |
1. Presence of two or more recognition sites
2. Presence of origin of replication
3. Presence of a marker gene
4. Presence of single restriction enzyme site
| 1. | The gene 'X' is responsible for controlling the copy number of the linked DNA and 'Y' for protein involved in the replication of Plasmid. |
| 2. | The gene 'X' is 'for protein involved in replication of Plasmid and 'Y' for resistance to antibiotics. |
| 3. | Gene 'X' is responsible for recognition sites and 'Y' is responsible for antibiotic resistance. |
| 4. | The gene 'X' is responsible for resistance to antibiotics and 'Y' for protein involved in the replication of Plasmid. |
In the above-represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?
| 1. | White color colonies will be selected. |
| 2. | Blue color colonies grown on ampicillin plates can be selected. |
| 3. | Using an ampicillin- & tetracycline-containing medium plate. |
| 4. | Blue color colonies will be selected. |
Preparing Cloning Vector for NEET
Cloning Vector is a specific sub-topic that frequently appears in the NEET examination. Understanding the underlying principles and practicing targeted questions is key to mastering this concept.
The questions compiled above are previous year questions (PYQs) directly related to Cloning Vector. Practicing these specific questions helps you understand the difficulty level and the examiner's approach to this topic.
Topic Frequently Asked Questions
Is Cloning Vector a high-weightage topic?
You can refer to the priority and consistency badges at the top of this page. High priority topics should be thoroughly revised multiple times before the exam.
Should I memorize the solutions?
No, it is highly recommended to understand the core concept and methodology behind each solution rather than memorizing them, as exact questions are rarely repeated, but the concepts definitely are.