Cloning Vector

Plasmid pBR322 has a PstI restriction enzyme site within gene amp^R that confers ampicillin resistance. If this enzyme is used for inserting a gene for $\mathrm{\backslash beta}$-galactoside production and the recombinant plasmid is inserted in an E.coli strain,

1.	it will lead to the lysis of host cells.
2.	it will be able to produce a novel protein with dual abilities.
3.	it will not be able to confer ampicillin resistance to the host cell.
4.	the transformed cells will have the ability to resist ampicillin as well as produce \beta -galactoside.

Which of the following is not a desirable feature of a cloning vector?
1. Presence of two or more recognition sites
2. Presence of origin of replication
3. Presence of a marker gene
4. Presence of single restriction enzyme site

The following diagram showing restriction sites in E.coli cloning vector pBR322. Find the role of 'X' and 'Y' genes :

1.	The gene 'X' is responsible for controlling the copy number of the linked DNA and 'Y' for protein involved in the replication of Plasmid.
2.	The gene 'X' is 'for protein involved in replication of Plasmid and 'Y' for resistance to antibiotics.
3.	Gene 'X' is responsible for recognition sites and 'Y' is responsible for antibiotic resistance.
4.	The gene 'X' is responsible for resistance to antibiotics and 'Y' for protein involved in the replication of Plasmid.

In the above-represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?

1.	White color colonies will be selected.
2.	Blue color colonies grown on ampicillin plates can be selected.
3.	Using an ampicillin- & tetracycline-containing medium plate.
4.	Blue color colonies will be selected.